Escherichia coli AKN10

Ancestors

Derived strains

Escherichia coli AKN11

Genotype with respect to parental

P_tac-purF

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Δzwf | P_tac-talA | purF^{K326Q−P410W} | prs^D128A | △purR | △pfkA | P_tac-prs | P_tac-purF

Bars (|) indicate differences between strains.

Modifications

Origin	Gene	Organism	Modification
Escherichia coli AKN3	zwf	Escherichia coli	Silencing
Escherichia coli AKN4	talA	Escherichia coli	Overexpression
Escherichia coli AKN5	purF	Escherichia coli	Overexpression
Escherichia coli AKN6	prsA	Escherichia coli	Feedback insensitive
Escherichia coli AKN7	purR	Escherichia coli	Silencing
Escherichia coli AKN8	pfkA	Escherichia coli	Silencing
Escherichia coli AKN9	prsA	Escherichia coli	Overexpression
Escherichia coli AKN10	purF	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
IMP	Titer	780.7 mg/L		Glucose		Flask	[341]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Li, Xiaoxi; Yu, Wenwen; Guo, Baoyuan; Lang, Xutao; Xu, Xianhao; Liu, Yanfeng; Li, Jianghua; Du, Guocheng; Lv, Xueqin & Liu, Long. Metabolic engineering of Escherichia coli for biosynthesis of inosinic acid. Systems Microbiology and Biomanufacturing. 2025, 5(4), 1609-1621.