Escherichia coli AKN14
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Derived strains
Genotype with respect to parental
ΔpurA
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Δzwf | Ptac-talA | purFK326Q−P410W | prsD128A | △purR | △pfkA | Ptac-prs | Ptac-purF | ΔnagD | △guaB | ΔpurABars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Overexpression
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Feedback insensitive
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| IMP | Titer | 723.4 mg/L | Flask | [341] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.