Escherichia coli AKN15
Ancestors
Derived strains
Genotype with respect to parental
PAICS::yncK
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Δzwf | Ptac-talA | purFK326Q−P410W | prsD128A | △purR | △pfkA | Ptac-prs | Ptac-purF | ΔnagD | △guaB | ΔpurA | PAICS::yncKBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Overexpression
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Feedback insensitive
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| IMP | Titer | 1491.2 mg/L | Flask | [341] | |||
| hypoxanthine | Titer | 644.7 mg/L | Flask | [341] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.