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Escherichia coli Glu-02

Ancestors
  • All strains
    • Escherichia coli FMME
      • Escherichia coli F0723
        • Escherichia coli Glu-02

Genotype with respect to wild type
ΔgdhA::gox-ΔcsiD::nox | Plasmid pJ07-gabP added | Plasmid pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22 added

Bars (|) indicate differences between strains.


Plasmids
  • pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22 (pBR322 ori, PTrc-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22-TT7)

    pJ07-gabP (ColE1 ori, PJ23119-gabP)


Modifications
Origin Gene Organism Modification
Escherichia coli F0723
gdhA
Escherichia coli
Silencing
Escherichia coli F0723
glaH
Escherichia coli
Silencing
pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22
cadA
Escherichia coli
Overexpression
pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22
patA
Klebsiella pneumoniae
Overexpression
pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22
KpcPD
Klebsiella pneumoniae
Overexpression
pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22
gabT
Pseudomonas fluorescens
Overexpression
pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22-PfeGTRBS22-PfeGDRBS22
gabD
Pseudomonas fluorescens
Overexpression
pJ07-gabP
gabP
Escherichia coli
Overexpression

References
  • Jiaping Wang, Cong Gao, Xiulai Chen & Liming Liu (2021). Engineering the Cad pathway in Escherichia coli to produce glutarate from l-lysine. Applied Microbiology and Biotechnology.

Reference

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