Escherichia coli AROM-3 (pQE-hpaBC)
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔtyrR, ΔcsrA | ΔptsHI, Δcrr | P37-galP-P37-glk | Plasmid pQE30-hpaBC addedBars (|) indicate differences between strains.
Plasmids
-
pQE30-hpaBC (PT5-hpaBC)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-dopa | Titer | 173.9 mg/L | 4.56 OD600 | Flask | [342] | ||
| L-tyrosine | Titer | 304.0 mg/L | 4.56 OD600 | Flask | [342] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.