Escherichia coli DOPA-30
This strain was generated using MAGE, although the specific final modifications were not disclosed.
Strain generated through random mutagenesis.
Ancestors
Derived strains
- Escherichia coli DOPA-30N
- Escherichia coli DOPA-30 (pQE30)
- Escherichia coli DOPA-30 (pQE30-2hpaBC)
- Escherichia coli DOPA-30 (pQE30-phaBCN)
Genotype with respect to parental
MAGE, artificial 5′-UTR sequence of the hpaC
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔtyrR, ΔcsrA | ΔptsHI, Δcrr | P37-galP-P37-glk | Δzwf | ΔpheLA | attPP21::7P37-tyrAfbr-tyrB-hpaBC fusion protein chimera | ΔmutS | MAGE, artificial 5′-UTR sequence of the hpaCBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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