Escherichia coli DOPA-30N
Strain derived from a randomly mutated strain.
Ancestors
Genotype with respect to parental
native 5′-UTR sequence of the hpaC
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔtyrR, ΔcsrA | ΔptsHI, Δcrr | P37-galP-P37-glk | Δzwf | ΔpheLA | attPP21::7P37-tyrAfbr-tyrB-hpaBC fusion protein chimera | ΔmutS | MAGE, artificial 5′-UTR sequence of the hpaC | native 5′-UTR sequence of the hpaCBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-dopa | Titer | 614.3 mg/L | 4.87 OD600 | Flask | [342] | ||
| L-tyrosine | Titer | 0.0 mg/L | 4.87 OD600 | Flask | [342] | ||
| L-dopa | Titer | 8.67 g/L | 110.0 OD600 | 60 h | Fed-Batch | [342] | |
| L-dopa | Volumetric productivity | 144.5 mg/L/h | 110.0 OD600 | 60 h | Fed-Batch | [342] | |
| L-dopa | Substrate yield | 62.7 mg/g of sustrate | 110.0 OD600 | 60 h | Fed-Batch | [342] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.