Escherichia coli DOPA-30 (pQE30)
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Strain derived from a randomly mutated strain.
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔtyrR, ΔcsrA | ΔptsHI, Δcrr | P37-galP-P37-glk | Δzwf | ΔpheLA | attPP21::7P37-tyrAfbr-tyrB-hpaBC fusion protein chimera | ΔmutS | MAGE, artificial 5′-UTR sequence of the hpaC | Plasmid pQE30 addedBars (|) indicate differences between strains.
Plasmids
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pQE30 (T5 promoter, pBR322 ori)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-dopa | Titer | 210.2 mg/L | 4.87 OD600 | Flask | [342] | ||
| L-tyrosine | Titer | 462.7 mg/L | 4.87 OD600 | Flask | [342] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.