Escherichia coli VH33_TIR

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli VH33

Escherichia coli VH33_TIR

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔptsI, ptsH, crr::kan ΔlacI, lacZ::loxP, P_galP::P_trc | Plasmid pJLBaroG^fbrtktA added | Plasmid pTrctyrCpheA_CM added

Bars (|) indicate differences between strains.

Plasmids

pJLBaroG^fbrtktA (lacI^q, P_lacUV5-aroG^fbr, P_tktA-tktA)

pTrctyrCpheA_CM (P_trc-tyrC-pheA_CM)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli VH33	ptsI	Escherichia coli	Silencing
Escherichia coli VH33	ptsH	Escherichia coli	Silencing
Escherichia coli VH33	crr	Escherichia coli	Silencing
Escherichia coli VH33	lacI	Escherichia coli	Silencing
Escherichia coli VH33	lacZ	Escherichia coli	Silencing
Escherichia coli VH33	galP	Escherichia coli	Overexpression
pJLBaroG^fbrtktA	aroG	Escherichia coli	Feedback insensitive
pJLBaroG^fbrtktA	tktA	Escherichia coli	Overexpression
pTrctyrCpheA_CM	tyrC	Zymomonas mobilis	Overexpression
pTrctyrCpheA_CM	pheA	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tyrosine	Titer	220.0 mg/L		Glucose		Flask	[343]
L-tyrosine	Substrate yield	118.0 mg/g of sustrate		Glucose		Flask	[343]
3-dehydroshikimate	Titer	134.0 mg/L		Glucose		Flask	[343]
shikimate	Titer	340.0 mg/L		Glucose		Flask	[343]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Muñoz, Ana Joyce; Hernández-Chávez, Georgina; de Anda, Ramon; Martínez, Alfredo; Bolívar, Francisco & Gosset, Guillermo. Metabolic engineering of Escherichia coli for improving l-3,4-dihydroxyphenylalanine (l-DOPA) synthesis from glucose. Journal of Industrial Microbiology & Biotechnology. 2011, 38(11), 1845-1852.