Escherichia coli W3110ΔtyrR_TIR
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtyrR::FRT | Plasmid pJLBaroGfbrtktA added | Plasmid pTrctyrCpheACM addedBars (|) indicate differences between strains.
Plasmids
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pJLBaroGfbrtktA (lacIq, PlacUV5-aroGfbr, PtktA-tktA)
pTrctyrCpheACM (Ptrc-tyrC-pheACM)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tyrosine | Titer | 220.0 mg/L | Flask | [343] | |||
| L-tyrosine | Substrate yield | 42.0 mg/g of sustrate | Flask | [343] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Muñoz, Ana Joyce; Hernández-Chávez, Georgina; de Anda, Ramon; Martínez, Alfredo; Bolívar, Francisco & Gosset, Guillermo. Metabolic engineering of Escherichia coli for improving l-3,4-dihydroxyphenylalanine (l-DOPA) synthesis from glucose. Journal of Industrial Microbiology & Biotechnology. 2011, 38(11), 1845-1852.