Escherichia coli W3110ΔtyrR_DOPA

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli W3110ΔtyrR

Escherichia coli W3110ΔtyrR_DOPA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtyrR::FRT | Plasmid pTrchpaBCTrctyrCpheA_CM added | Plasmid pJLBaroG^fbrtktA added

Bars (|) indicate differences between strains.

Plasmids

pJLBaroG^fbrtktA (lacI^q, P_lacUV5-aroG^fbr, P_tktA-tktA)

pTrchpaBCTrctyrCpheA_CM (P_trc-hpaBC, P_trc-tyrC-pheA_CM)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli W3110ΔtyrR	tyrR	Escherichia coli	Silencing
pJLBaroG^fbrtktA	aroG	Escherichia coli	Feedback insensitive
pJLBaroG^fbrtktA	tktA	Escherichia coli	Overexpression
pTrchpaBCTrctyrCpheA_CM	hpaB	Escherichia coli	Overexpression
pTrchpaBCTrctyrCpheA_CM	hpaC	Escherichia coli	Overexpression
pTrchpaBCTrctyrCpheA_CM	tyrC	Zymomonas mobilis	Overexpression
pTrchpaBCTrctyrCpheA_CM	pheA	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tyrosine	Titer	60.0 mg/L		Glucose		Flask	[343]
L-tyrosine	Titer	62.0 mg/L		Glucose		Flask	[343]
shikimate	Titer	248.0 mg/L		Glucose		Flask	[343]
shikimate	Titer	228.0 mg/L		Glucose		Flask	[343]
3-dehydroshikimate	Titer	0.0 mg/L		Glucose		Flask	[343]
3-dehydroshikimate	Titer	0.0 mg/L		Glucose		Flask	[343]
L-dopa	Titer	235.0 mg/L		Glucose		Flask	[343]
L-dopa	Titer	269.0 mg/L		Glucose		Flask	[343]
L-dopa	Substrate yield	35.0 mg/g of sustrate		Glucose		Flask	[343]
L-dopa	Substrate yield	41.0 mg/g of sustrate		Glucose		Flask	[343]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Muñoz, Ana Joyce; Hernández-Chávez, Georgina; de Anda, Ramon; Martínez, Alfredo; Bolívar, Francisco & Gosset, Guillermo. Metabolic engineering of Escherichia coli for improving l-3,4-dihydroxyphenylalanine (l-DOPA) synthesis from glucose. Journal of Industrial Microbiology & Biotechnology. 2011, 38(11), 1845-1852.