Escherichia coli VH33ΔtyrR_DOPA

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli VH33

Escherichia coli VH33ΔtyrR

Escherichia coli VH33ΔtyrR_DOPA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔptsI, ptsH, crr::kan ΔlacI, lacZ::loxP, P_galP::P_trc | ΔtyrR::FRT | Plasmid pTrchpaBCTrctyrCpheA_CM added | Plasmid pJLBaroG^fbrtktA added

Bars (|) indicate differences between strains.

Plasmids

pJLBaroG^fbrtktA (lacI^q, P_lacUV5-aroG^fbr, P_tktA-tktA)

pTrchpaBCTrctyrCpheA_CM (P_trc-hpaBC, P_trc-tyrC-pheA_CM)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli VH33	ptsI	Escherichia coli	Silencing
Escherichia coli VH33	ptsH	Escherichia coli	Silencing
Escherichia coli VH33	crr	Escherichia coli	Silencing
Escherichia coli VH33	lacI	Escherichia coli	Silencing
Escherichia coli VH33	lacZ	Escherichia coli	Silencing
Escherichia coli VH33	galP	Escherichia coli	Overexpression
Escherichia coli VH33ΔtyrR	tyrR	Escherichia coli	Silencing
pJLBaroG^fbrtktA	aroG	Escherichia coli	Feedback insensitive
pJLBaroG^fbrtktA	tktA	Escherichia coli	Overexpression
pTrchpaBCTrctyrCpheA_CM	hpaB	Escherichia coli	Overexpression
pTrchpaBCTrctyrCpheA_CM	hpaC	Escherichia coli	Overexpression
pTrchpaBCTrctyrCpheA_CM	tyrC	Zymomonas mobilis	Overexpression
pTrchpaBCTrctyrCpheA_CM	pheA	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tyrosine	Titer	51.0 mg/L		Glucose		Flask	[343]
L-tyrosine	Titer	22.0 mg/L		Glucose		Flask	[343]
shikimate	Titer	226.0 mg/L		Glucose		Flask	[343]
shikimate	Titer	271.0 mg/L		Glucose		Flask	[343]
3-dehydroshikimate	Titer	225.0 mg/L		Glucose		Flask	[343]
3-dehydroshikimate	Titer	312.0 mg/L		Glucose		Flask	[343]
L-dopa	Titer	313.0 mg/L		Glucose		Flask	[343]
L-dopa	Titer	320.0 mg/L		Glucose		Flask	[343]
L-dopa	Substrate yield	54.0 mg/g of sustrate		Glucose		Flask	[343]
L-dopa	Substrate yield	54.0 mg/g of sustrate		Glucose		Flask	[343]
L-dopa	Titer	1.51 g/L	6.75 g/L	Glucose	40 h	Batch	[343]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Muñoz, Ana Joyce; Hernández-Chávez, Georgina; de Anda, Ramon; Martínez, Alfredo; Bolívar, Francisco & Gosset, Guillermo. Metabolic engineering of Escherichia coli for improving l-3,4-dihydroxyphenylalanine (l-DOPA) synthesis from glucose. Journal of Industrial Microbiology & Biotechnology. 2011, 38(11), 1845-1852.