Escherichia coli R21
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | ΔsroG, ribFT203D | ΔpfkA | Δedd, Δeda | ΔyajO, purR::pgl | yghX::ribM | Plasmid pNEW-AZ addedBars (|) indicate differences between strains.
Plasmids
-
pNEW-AZ (ribC, ribE, ribB, ribD, ribA, zwf)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Attenuation
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Overexpression
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| riboflavin | Titer | 945.68 mg/L | 4.8 OD600 | Flask | [345] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.