Escherichia coli DH5αZ1 (pZE-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)

Ancestors

Genotype with respect to wild type

lacI^q, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 | Plasmid pZE-wvds added | Plasmid pBbA5c-MevT(CO)-MBIS(CO, ispA) added

Bars (|) indicate differences between strains.

Plasmids

• pZE-wvds (vds^WT)

  pBbA5c-MevT(CO)-MBIS(CO, ispA) (p15A ori, P_trc, atoB, hmgs, hmgr, erg12, erg8, mvd1, idi, and ispA)

References

• Nybo, S. Eric; Saunders, Jacqueline & McCormick, Sean P. Metabolic engineering of Escherichia coli for production of valerenadiene. Journal of Biotechnology. 2017, 262, 60-66.