Escherichia coli DH5αZ1 (pZE-cvds)

Ancestors

Derived strains

Genotype with respect to wild type

lacI^q, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 | Plasmid pZE-cvds added

Bars (|) indicate differences between strains.

Plasmids

pZE-cvds (vds^Opt)

Modifications

Origin	Gene	Organism	Modification
pZE-cvds	vds	Valeriana officinalis	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
valerena-4,7(11)-diene	Titer	16.0 µg/L		Yeast extract Tryptone	24 h	Flask	[346]
valerena-4,7(11)-diene	Titer	32.0 µg/L		Yeast extract Tryptone	48 h	Flask	[346]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Nybo, S. Eric; Saunders, Jacqueline & McCormick, Sean P. Metabolic engineering of Escherichia coli for production of valerenadiene. Journal of Biotechnology. 2017, 262, 60-66.