Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps)

Ancestors

Genotype with respect to wild type

lacI^q, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 | Plasmid pZE-cvds added | Plasmid pSTV-dxs-idi-fps added

Bars (|) indicate differences between strains.

Plasmids

pZE-cvds (vds^Opt)

pSTV-dxs-idi-fps (dxs-idi-fps)

Modifications

Origin	Gene	Organism	Modification
pZE-cvds	vds	Valeriana officinalis	Overexpression
pSTV-dxs-idi-fps	dxs	Escherichia coli	Overexpression
pSTV-dxs-idi-fps	idi	Escherichia coli	Overexpression
pSTV-dxs-idi-fps	fps	Gallus gallus	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
valerena-4,7(11)-diene	Titer	2.09 mg/L		Yeast extract Tryptone	48 h	Flask	[346]
valerena-4,7(11)-diene	Titer	11.0 mg/L		Glycerol	48 h	Flask	[346]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Nybo, S. Eric; Saunders, Jacqueline & McCormick, Sean P. Metabolic engineering of Escherichia coli for production of valerenadiene. Journal of Biotechnology. 2017, 262, 60-66.