Escherichia coli DH5αZ1 (pZE-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)

Ancestors

Genotype with respect to wild type

lacI^q, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 | Plasmid pZE-cvds added | Plasmid pBbA5c-MevT(CO)-MBIS(CO, ispA) added

Bars (|) indicate differences between strains.

Plasmids

pZE-cvds (vds^Opt)

pBbA5c-MevT(CO)-MBIS(CO, ispA) (p15A ori, P_trc, atoB, hmgs, hmgr, erg12, erg8, mvd1, idi, and ispA)

Modifications

Origin	Gene	Organism	Modification
pZE-cvds	vds	Valeriana officinalis	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	atoB	Escherichia coli	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	ERG13	Saccharomyces cerevisiae	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	HMG1	Saccharomyces cerevisiae	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	ERG12	Saccharomyces cerevisiae	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	ERG8	Saccharomyces cerevisiae	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	MVD1	Saccharomyces cerevisiae	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	idi	Escherichia coli	Overexpression
pBbA5c-MevT(CO)-MBIS(CO, ispA)	ispA	Escherichia coli	Overexpression

References

Nybo, S. Eric; Saunders, Jacqueline & McCormick, Sean P. Metabolic engineering of Escherichia coli for production of valerenadiene. Journal of Biotechnology. 2017, 262, 60-66.