Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
Ancestors
Genotype with respect to wild type
lacIq, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 | Plasmid pTrcHis-cvds added | Plasmid pBbA5c-MevT(CO)-MBIS(CO, ispA) addedBars (|) indicate differences between strains.
Plasmids
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pTrcHis-cvds (vdsOpt)
pBbA5c-MevT(CO)-MBIS(CO, ispA) (p15A ori, Ptrc, atoB, hmgs, hmgr, erg12, erg8, mvd1, idi, and ispA)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| valerena-4,7(11)-diene | Titer | 42.5 mg/L | 48 h | Flask | [346] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.