Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pET-cvds added | Plasmid pBbA5c-MevT(CO)-MBIS(CO, ispA) addedBars (|) indicate differences between strains.
Plasmids
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pET-cvds (PT7-vdsOpt-His-Tag)
pBbA5c-MevT(CO)-MBIS(CO, ispA) (p15A ori, Ptrc, atoB, hmgs, hmgr, erg12, erg8, mvd1, idi, and ispA)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| valerena-4,7(11)-diene | Titer | 62.0 mg/L | 48 h | Flask | [346] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.