Escherichia coli p15aT5 + metK

Ancestors

Genotype with respect to wild type

recA1, thi, endA1, supE44, gyrA96, hsdR17 Δ(lac-proAB)/(lac-proAB)/F′[traD36,proAB+ , lacIq , lacZΔM15] | (DE3) | Plasmid p15a-T5-tal-sam5-comt added | Plasmid pCL1920-T7-SsmetK added

Bars (|) indicate differences between strains.

Plasmids

p15a-T5-tal-sam5-comt (p15a ori, P_T5-tal-sam5-comt-T_T7)

pCL1920-T7-SsmetK (P_T7-SsmetK-TT7)

Modifications

Origin	Gene	Organism	Modification
p15a-T5-tal-sam5-comt	hutH	Rhodobacter sphaeroides	Overexpression
p15a-T5-tal-sam5-comt	sam5	Saccharothrix espanaensis	Overexpression
p15a-T5-tal-sam5-comt	comt	Triticum aestivum	Overexpression
pCL1920-T7-SsmetK	metK	Streptomyces spectabilis	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
ferulate	Titer	197.8 mg/L ^*		L-tyrosine		Flask	[348]
trans-caffeate	Titer	6.2 mg/L ^*		L-tyrosine		Flask	[348]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Lv, Huajun; Zhang, Ying; Shao, Jie; Liu, Haili & Wang, Yong. Ferulic acid production by metabolically engineered Escherichia coli. Bioresources and Bioprocessing. 2021, 8(1).