Escherichia coli T5FA + pCL1920-T7

This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.

Synonyms

Escherichia coli T5FA pSC101-T7

Ancestors

All strains

Escherichia coli JM109

Escherichia coli JM109 (DE3)

Escherichia coli T5FA

Escherichia coli T5FA + pCL1920-T7

Genotype with respect to wild type

recA1, thi, endA1, supE44, gyrA96, hsdR17 Δ(lac-proAB)/(lac-proAB)/F′[traD36,proAB+ , lacIq , lacZΔM15] | (DE3) | Plasmid pBR322-T5-tal-sam5-comt added | Plasmid pCL1920-T7 added

Bars (|) indicate differences between strains.

Plasmids

pBR322-T5-tal-sam5-comt (P_T5-tal-sam5-comt)

pCL1920-T7 (P_T7-MCS-T_T7)

Modifications

Origin	Gene	Organism	Modification
pBR322-T5-tal-sam5-comt	hutH	Rhodobacter sphaeroides	Overexpression
pBR322-T5-tal-sam5-comt	sam5	Saccharothrix espanaensis	Overexpression
pBR322-T5-tal-sam5-comt	comt	Triticum aestivum	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
ferulate	Titer	140.0 mg/L ^*		L-tyrosine		Flask	[348]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Lv, Huajun; Zhang, Ying; Shao, Jie; Liu, Haili & Wang, Yong. Ferulic acid production by metabolically engineered Escherichia coli. Bioresources and Bioprocessing. 2021, 8(1).