Escherichia coli T5FA + zwf
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
recA1, thi, endA1, supE44, gyrA96, hsdR17 Δ(lac-proAB)/(lac-proAB)/F′[traD36,proAB+ , lacIq , lacZΔM15] | (DE3) | Plasmid pBR322-T5-tal-sam5-comt added | Plasmid pCL1920-T7-Eczwf addedBars (|) indicate differences between strains.
Plasmids
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pBR322-T5-tal-sam5-comt (PT5-tal-sam5-comt)
pCL1920-T7-Eczwf (PT7-Eczwf-TT7)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| ferulate | Titer | 133.89 mg/L * | Flask | [348] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.