Escherichia coli CUR02 pYk-CUS M1-4AT/pTrc99a-Almgs
The supplementation of palmitoleic acid markedly enhances curcumin biosynthesis, likely by improving membrane fluidity and thereby facilitating the metabolic flux toward lipophilic compound formation [349].
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔpoxB ::acs ΔadhE | ΔfabF | ΔcurA | Plasmid pYk-CUS M1-4AT added | Plasmid pTrc99a-Almgs addedBars (|) indicate differences between strains.
Plasmids
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pYk-CUS M1-4AT (Mutant p15A ori, PBAD-CUS M1-4AT)
pTrc99a-Almgs (mgsAl)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| curcumin | Titer | 0.25 mM * | 12 h | Flask | [349] | ||
| curcumin | Titer | 0.357 mM * | 12 h | Flask | [349] | ||
| curcumin | Titer | 0.373 mM * | 12 h | Flask | [349] | ||
| curcumin | Titer | 0.435 mM * | 12 h | Flask | [349] | ||
| curcumin | Titer | 0.407 mM * | 12 h | Flask | [349] | ||
| curcumin | Titer | 0.377 mM * | 12 h | Flask | [349] | ||
| curcumin | Titer | 1.46 mM | 48 h | Flask | [349] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.