Escherichia coli B-AP5

Ancestors

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pE-FNS added | Plasmid pC-pPeCHS-Os4CL-pMtCHI added | Plasmid pA-aroG-SeTAL-tyrA added

Bars (|) indicate differences between strains.

Plasmids

pC-pPeCHS-Os4CL-pMtCHI (P_T7-CHS_Pe-4CL_Os, P_T7-CHI_Mt)

pE-FNS (FNS_Pc)

pA-aroG-SeTAL-tyrA (TAL_Se, aroG_Ec, tyrA_Ec)

Modifications

Origin	Gene	Organism	Modification
pC-pPeCHS-Os4CL-pMtCHI	4CL	Oryza sativa	Overexpression
pC-pPeCHS-Os4CL-pMtCHI	CHS	Populus euramericana	Overexpression
pC-pPeCHS-Os4CL-pMtCHI	CHI	Medicago truncatula	Overexpression
pE-FNS	FNS	Petroselinum crispum	Overexpression
pA-aroG-SeTAL-tyrA	sam8	Saccharothrix espanaensis	Overexpression
pA-aroG-SeTAL-tyrA	aroG	Escherichia coli	Overexpression
pA-aroG-SeTAL-tyrA	tyrA	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
genkwanin	Titer	10.0 mg/L		Glucose		Flask	[351]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Lee, Hyejin; Kim, Bong Gyu; Kim, Mihyang & Ahn, Joong-Hoon (2015). Biosynthesis of Two Flavones, Apigenin and Genkwanin, in Escherichia coli. Journal of Microbiology and Biotechnology, 25(9), 1442-1448.