Escherichia coli B-GK

Ancestors

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pE-POMT7-FNS added | Plasmid pC-pPeCHS-Os4CL-pMtCHI added | Plasmid pA-aroG^fbr-SeTAL-tyrA^fbr added

Bars (|) indicate differences between strains.

Plasmids

pC-pPeCHS-Os4CL-pMtCHI (P_T7-CHS_Pe-4CL_Os, P_T7-CHI_Mt)

pE-POMT7-FNS (POMT7_Pd, FNS_Pc)

pA-aroG^fbr-SeTAL-tyrA^fbr (TAL_Se, aroG^fbr_Ec, tyrA^fbr_Ec)

Modifications

Origin	Gene	Organism	Modification
pC-pPeCHS-Os4CL-pMtCHI	4CL	Oryza sativa	Overexpression
pC-pPeCHS-Os4CL-pMtCHI	CHS	Populus euramericana	Overexpression
pC-pPeCHS-Os4CL-pMtCHI	CHI	Medicago truncatula	Overexpression
pE-POMT7-FNS	FNS	Petroselinum crispum	Overexpression
pE-POMT7-FNS	POMT7	Populus deltoids	Overexpression
pA-aroG^fbr-SeTAL-tyrA^fbr	sam8	Saccharothrix espanaensis	Overexpression
pA-aroG^fbr-SeTAL-tyrA^fbr	aroG	Escherichia coli	Feedback insensitive
pA-aroG^fbr-SeTAL-tyrA^fbr	tyrA	Escherichia coli	Feedback insensitive

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
genkwanin	Titer	41.0 mg/L		Glucose	30 h	Flask	[351]
apigenin	Titer	55.0 mg/L		Glucose	30 h	Flask	[351]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Lee, Hyejin; Kim, Bong Gyu; Kim, Mihyang & Ahn, Joong-Hoon (2015). Biosynthesis of Two Flavones, Apigenin and Genkwanin, in Escherichia coli. Journal of Microbiology and Biotechnology, 25(9), 1442-1448.