Escherichia coli GA04
This strain produced 190 mg/L of the by-products 3-methyl-crotonate (3-MC) and 3-methyl-3-butanoate (3-MB).
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔrecA ΔendA (DE3) | Plasmid pIG_01 added | Plasmid pGA04 addedBars (|) indicate differences between strains.
Plasmids
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pIG_01 (pMB1 ori, PBAD-EcthiM-MvIPK-Ecidi, PT7-ObgeS(truncated)-Aggpps)
pGA04 (pSC101 ori, PthrC3-CdGeDH-CdGaDH)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| geranate | Titer | 661.0 mg/L * | Flask | [354] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.