Escherichia coli DSM01‐BDO

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | fhuA2 | △frdA::FRT△pta::FRT△ldhA::FRT△adhE::FRT | Plasmid pZS‐BDO added

Bars (|) indicate differences between strains.


Plasmids

Modifications
Origin Gene Organism Modification
Silencing
pta
Silencing
Silencing
Silencing
Overexpression
Overexpression
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
(R,R)-2,3-butanediol Titer 2.45 g/L * Flask [356]
(R,R)-2,3-butanediol Titer 3.59 g/L * 48 h * Batch [356]
(R,R)-2,3-butanediol Titer 10.09 g/L * 48 h * Batch [356]
(R,R)-2,3-butanediol Titer 17.71 g/L * 48 h * Batch [356]
(R,R)-2,3-butanediol Substrate yield 0.113 g/g of sustrate * 48 h * Batch [356]
(R,R)-2,3-butanediol Substrate yield 0.21 g/g of sustrate * 48 h * Batch [356]
(R,R)-2,3-butanediol Substrate yield 0.312 g/g of sustrate * 48 h * Batch [356]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References