Escherichia coli ETC4‐BDO
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | fhuA2 | △frdA::FRT△pta::FRT△ldhA::FRT△adhE::FRT | △nuo △cyo △cbd | Plasmid pZS‐BDO addedBars (|) indicate differences between strains.
Plasmids
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pZS‐BDO (budABCEa)
Modifications
| Origin | Gene | Organism | Modification |
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| (R,R)-2,3-butanediol | Titer | 5.2 g/L * | Flask | [356] | |||
| (R,R)-2,3-butanediol | Titer | 13.7 g/L * | 48 h | Batch | [356] | ||
| (R,R)-2,3-butanediol | Titer | 14.73 g/L * | 48 h | Batch | [356] | ||
| (R,R)-2,3-butanediol | Titer | 18.43 g/L * | 48 h | Batch | [356] | ||
| (R,R)-2,3-butanediol | Titer | 14.8 g/L * | 36 h * | Batch | [356] | ||
| (R,R)-2,3-butanediol | Substrate yield | 0.243 g/g of sustrate * | 48 h * | Batch | [356] | ||
| (R,R)-2,3-butanediol | Substrate yield | 0.267 g/g of sustrate * | 48 h * | Batch | [356] | ||
| (R,R)-2,3-butanediol | Substrate yield | 0.363 g/g of sustrate * | 48 h * | Batch | [356] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.