Escherichia coli KW013
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Strain derived from a randomly mutated strain.
Ancestors
Genotype with respect to parental
M1-12::glk
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔtrpR ΔtrpL | ΔpykF | ΔptsH | M1-12::galP | Plasmid PH5a1 lost | M1-12::glk | Plasmid PH5a1 addedBars (|) indicate differences between strains.
Plasmids
-
PH5a1 (aroGfbr trpEDCBA)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 0.59 g/L | Flask | [135] | |||
| L-tryptophan | Substrate yield | 0.13 g/g of sustrate | Flask | [135] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.