Escherichia coli BMF2

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21

Escherichia coli BL21(DE3)

Escherichia coli BMF2

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pRSF-acs-ACC added | Plasmid pET-CHS-CHI added | Plasmid pCDF-TAL-4CL added | Plasmid pACYC-tF3′H-tCPR added

Bars (|) indicate differences between strains.

Plasmids

pCDF-TAL-4CL (TAL_Rg, 4CL_Pc)

pET-CHS-CHI (CHI_Ms, CHS_Ph)

pRSF-acs-ACC (acs_Ec, accBC_Cg, dtsR1_Cg)

pACYC-tF3′H-tCPR (tF3′H_Gh-tCPR_Cs)

Modifications

Origin	Gene	Organism	Modification
pCDF-TAL-4CL	TAL	Rhodotorula glutinis	Overexpression
pCDF-TAL-4CL	4CL	Petroselinum crispum	Overexpression
pET-CHS-CHI	CHI1	Medicago sativa	Overexpression
pET-CHS-CHI	chs	Petunia hybrida	Overexpression
pRSF-acs-ACC	accBC	Corynebacterium glutamicum	Overexpression
pRSF-acs-ACC	dtsR1	Corynebacterium glutamicum	Overexpression
pRSF-acs-ACC	acs	Escherichia coli	Overexpression
pACYC-tF3′H-tCPR	F3'H	Gerbera hybrida	Overexpression
pACYC-tF3′H-tCPR	CPR	Catharanthus roseus	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
(2S)-eriodictyol	Titer	5.7 mg/L		Glucose		Flask	[359]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Zhu, Saijie; Wu, Junjun; Du, Guocheng; Zhou, Jingwen & Chen, Jian (2014). Efficient Synthesis of Eriodictyol from l -Tyrosine in Escherichia coli. Applied and Environmental Microbiology, 80(10), 3072-3080.