Escherichia coli TH07 (pBRThrABC, pACYC177)
In this strain, the impact of the empty vector is evaluated.
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | thrAfbr | lysCfbr | Pthr::Ptac | ΔlysA | ΔmetA | ilvAC290T | Δtdh | Plasmid pACYC177 added | Plasmid pBRThrABC addedBars (|) indicate differences between strains.
Plasmids
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Attenuation
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-threonine | Titer | 1.46 g/L | 5.88 g/L | 12 h | Batch | [190] | |
| L-threonine | Substrate yield | 0.14 g/g of sustrate | 5.88 g/L | 12 h | Batch | [190] | |
| acetate | Titer | 0.64 g/L | 5.88 g/L | 12 h | Batch | [190] | |
| succinate | Titer | 0.01 g/L | 5.88 g/L | 12 h | Batch | [190] | |
| pyruvate | Titer | 0.12 g/L | 5.88 g/L | 12 h | Batch | [190] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.