Escherichia coli TH27C (pBRThrABCR)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | thrAfbr | lysCfbr | Pthr::Ptac | ΔlysA | ΔmetA | ilvAC290T | Δtdh | iclR::CmR | Pppc::Ptrc | tdcC::CmR | Plasmid pBRThrABCR addedBars (|) indicate differences between strains.
Plasmids
-
pBRThrABCR (thrABC rhtC)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Attenuation
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Silencing
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Silencing
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Overexpression
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Silencing
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Overexpression
|
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Overexpression
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Overexpression
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Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-threonine | Substrate yield | 0.37 g/g of sustrate | 11.32 g/L | 14 h | Batch | [190] | |
| L-threonine | Titer | 11.1 g/L | 11.32 g/L | 14 h | Batch | [190] | |
| acetate | Titer | 0.8 g/L | 11.32 g/L | 14 h | Batch | [190] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.