Escherichia coli TS-7
Serine accumulation in this strain inhibits the threonine biosynthetic pathway, thereby impairing cellular growth and diminishing tryptophan production.
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Strain derived from a randomly mutated strain.
Ancestors
Derived strains
Genotype with respect to parental
serAmut
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔglnA::BSglnA | ΔackA::J23119-icd ΔpoxB::J23119-gdhA | ΔatpI::Ptrc::prs | serAmutBars (|) indicate differences between strains.
Plasmids
-
PH5a1 (aroGfbr trpEDCBA)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Feedback insensitive
|
|||
|
Feedback insensitive
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 2-oxoglutarate | Titer | 1.241 g/L | 48 h | Flask | [136] | ||
| acetate | Titer | 0.276 g/L | 48 h | Flask | [136] | ||
| L-tryptophan | Titer | 1.04 g/L | 48 h | Flask | [136] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.