Escherichia coli UR6
Ancestors
Genotype with respect to parental
△nupC, △nupG
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △lacI, yghX::Ptrc-pyrB-pyrC-pyrAA-pyrABfbr(E949*)-pyrK-pyrD-pyrF-pyrE | Δudk | Δudp | △rihA | △rihB | △rihC | tyrR::Ptrc-pyrAA-pyrAB | △argF | Pppc::Ptrc, △thrA, △iclR | △purR | trpR::prs | △nupC, △nupGBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Overexpression
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Silencing
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Silencing
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| uridine | Titer | 14.5 g/L | 33.6 OD600 | 32 h | Flask | [202] | |
| uridine | Substrate yield | 0.161 g/g of sustrate | 33.6 OD600 | 32 h | Flask | [202] | |
| uracil | Titer | 0.52 g/L | 33.6 OD600 | 32 h | Flask | [202] | |
| uridine | Titer | 70.3 g/L | 68.0 OD600 | 64 h | Fed-Batch | [202] | |
| uridine | Substrate yield | 0.259 g/g of sustrate | 68.0 OD600 | 64 h | Fed-Batch | [202] | |
| uridine | Volumetric productivity | 1.1 g/L/h | 68.0 OD600 | 64 h | Fed-Batch | [202] | |
| uracil | Titer | 0.9 g/L | 68.0 OD600 | 64 h | Fed-Batch | [202] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.