Escherichia coli HW3-2
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to parental
yeeP::Ptrc-zwf
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △lacI, hisL::Ptrc | △hisG, tdcD::Ptrc-hisGCgS143F | ylbE::Ptrc-hisGCgS143F | ilvG::Ptrc-hisGCgS143F | yghX::Ptrc-hisD-hisC-hisB-hisH-hisA-hisF-hisI | trpR::prsA, △purR | yeeP::Ptrc-zwfBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Feedback insensitive
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Silencing
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Feedback insensitive
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-histidine | Titer | 4.0 g/L | 36.5 OD600 | 28 h | Flask | [203] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.