Escherichia coli HW4-2
Ancestors
Derived strains
Genotype with respect to parental
rph::Ptrc-purH
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △lacI, hisL::Ptrc | △hisG, tdcD::Ptrc-hisGCgS143F | ylbE::Ptrc-hisGCgS143F | ilvG::Ptrc-hisGCgS143F | yghX::Ptrc-hisD-hisC-hisB-hisH-hisA-hisF-hisI | trpR::prsA, △purR | yeeP::Ptrc-zwf, yjiV::Ptrc-gnd | △mazG, △ushA, △surE, △yjjG, △yrfG, △amn, △ygdH | rph::Ptrc-purHBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Feedback insensitive
|
|||
|
Silencing
|
|||
|
Feedback insensitive
|
|||
|
Silencing
|
|||
|
Feedback insensitive
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Silencing
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-histidine | Titer | 5.6 g/L | 24.2 OD600 | Flask | [203] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.