Escherichia coli HW6-3
Ancestors
Genotype with respect to parental
yjiT::Ptrc-lysECg
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △lacI, hisL::Ptrc | △hisG, tdcD::Ptrc-hisGCgS143F | ylbE::Ptrc-hisGCgS143F | ilvG::Ptrc-hisGCgS143F | yghX::Ptrc-hisD-hisC-hisB-hisH-hisA-hisF-hisI | trpR::prsA, △purR | yeeP::Ptrc-zwf, yjiV::Ptrc-gnd | △mazG, △ushA, △surE, △yjjG, △yrfG, △amn, △ygdH | rph::Ptrc-purH | ycjV::Ptrc-purA | yjiP::Ptrc-purB | △purF | yjhE::Ptrc-rocG | yjiT::Ptrc-lysECgBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Feedback insensitive
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Silencing
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Feedback insensitive
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Overexpression
|
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Silencing
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
|
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Silencing
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Silencing
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Overexpression
|
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Silencing
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Overexpression
|
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|
Silencing
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Silencing
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Overexpression
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Silencing
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-histidine | Titer | 11.8 g/L | Flask | [203] | |||
| L-histidine | Titer | 66.5 g/L | 44 h | Fed-Batch | [203] | ||
| L-histidine | Volumetric productivity | 1.5 g/L/h | 44 h | Fed-Batch | [203] | ||
| L-histidine | Substrate yield | 0.23 g/g of sustrate | 44 h | Fed-Batch | [203] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.