Escherichia coli BL21(DE3)/pCTSDT
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pCTSDT addedBars (|) indicate differences between strains.
Plasmids
-
pCTSDT (PT7-ilvCEc-TT7-PT7-alsSBs-PT7-ilvDEc-TT7)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 3-methyl-2-oxobutanoate | Titer | 1.7 g/L | Flask | [221] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.