Escherichia coli 050T4/pCTSDTQ487S-RBS55
Ancestors
Genotype with respect to wild type
Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ilvE::T7RNAP | ΔleuA | PpntA::PT7 | Plasmid pCTSDTQ487S-RBS55 addedBars (|) indicate differences between strains.
Plasmids
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pCTSDTQ487S-RBS55 (PT7-ilvC-TT7-PT7-RBS55-alsSQ487S-PT7-ilvD)
Modifications
| Origin | Gene | Organism | Modification |
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 3-methyl-2-oxobutanoate | Titer | 19.2 g/L | Flask | [221] | |||
| isobutanol | Titer | 0.57 g/L | Flask | [221] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.