Escherichia coli XA05

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli BW25113/F'

Escherichia coli XY01

Escherichia coli XY02

Escherichia coli XY05

Escherichia coli XA05

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F׳ proAB lacI^qZΔM15 Tn10 (Tet^r) | ΔompT::P_tac-ppc_Cg | ΔpykA ΔpykF | Δmqo | Plasmid pLB1a-EC added

Bars (|) indicate differences between strains.

Plasmids

pLB1a-EC (P_araBAD-aspC)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli XY01	ompT	Escherichia coli	Silencing
Escherichia coli XY01	ppc	Corynebacterium glutamicum	Overexpression
Escherichia coli XY02	pykA	Escherichia coli	Silencing
Escherichia coli XY02	pykF	Escherichia coli	Silencing
Escherichia coli XY05	mqo	Escherichia coli	Silencing
pLB1a-EC	aspC	Escherichia coli	Overexpression

References

Xiaoyu Piao, Lei Wang, Baixue Lin, Hao Chen, Weifeng Liu & Yong Tao (2019). Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield. Metabolic Engineering.