Escherichia coli XZ123
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Strain generated through random mutagenesis.
Ancestors
Derived strains
Genotype with respect to parental
Metabolic evolution in NBS medium with 8% glucose
Genotype with respect to wild type
ΔpflB ΔfrdABCD ΔadhE ΔackA | ldhA::FRT-tet-FRT::alaDGs | Δtet | Metabolic evolution in NBS medium with 2% glucose | Metabolic evolution in NBS medium with 5% glucose | Metabolic evolution in NBS medium with 8% glucose | ΔmgsA | Metabolic evolution in NBS medium with 8% glucoseBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
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Silencing
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Overexpression
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Silencing
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-alanine | Titer | 832.0 mM | 48 h | Batch | [208] | ||
| L-alanine | Substrate yield | 93.0 % | 48 h | Batch | [208] | ||
| succinate | Titer | 2.0 mM | 48 h | Batch | [208] | ||
| acetate | Titer | 9.0 mM | 48 h | Batch | [208] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.