Escherichia coli FB-04(pta1)

Ancestors

Derived strains

Genotype with respect to parental

Δpta::pta1


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtyrA::FRT | Δpta::pta1 | Plasmid pSV03 added

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Origin Gene Organism Modification
Silencing
Overexpression
Overexpression
Overexpression
Overexpression
Overexpression
Silencing
Silencing
Silencing
pta
Attenuation
Feedback insensitive
Feedback insensitive
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 2.09 g/L 7.5 OD600 48 h Flask [146]
L-tryptophan Substrate yield 0.049 g/g of sustrate 7.5 OD600 48 h Flask [146]
L-tryptophan Volumetric productivity 0.044 g/L/h 7.5 OD600 48 h Flask [146]
acetate Titer 8.4 g/L 7.5 OD600 48 h Flask [146]
acetate Titer 2.1 g/L 82.8 OD600 54 h Fed-Batch [146]
L-tryptophan Titer 44.0 g/L 82.8 OD600 54 h Fed-Batch [146]
L-tryptophan Substrate yield 0.13 g/g of sustrate 82.8 OD600 54 h Fed-Batch [146]
L-tryptophan Volumetric productivity 0.82 g/L/h 82.8 OD600 54 h Fed-Batch [146]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References