Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH)

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | thrA^fbr | lysC^fbr | P_thr::P_tac | ΔlysA | ΔmetA | ilvA^C290T | Δtdh | iclR::Cm^R | P_ppc::P_trc | ΔCm^R | ilvA^{C1339T, G1341T, C1351G, T1352C} | P_ilvEDA::P_trc, P_ilvC::P_trc | P_lrp::P_trc | Plasmid pBRthrABCygaZH added | Plasmid pTacilvAIH added

Bars (|) indicate differences between strains.

Plasmids

pTacilvAIH (ilvAIH)

pBRthrABCygaZH (thrA^fbrBC ygaZH)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli WL3110	lacI	Escherichia coli	Silencing
Escherichia coli TH01	thrA	Escherichia coli	Feedback insensitive
Escherichia coli TH02	lysC	Escherichia coli	Feedback insensitive
Escherichia coli TH03	thrA	Escherichia coli	Overexpression
Escherichia coli TH03	thrB	Escherichia coli	Overexpression
Escherichia coli TH03	thrC	Escherichia coli	Overexpression
Escherichia coli TH04	lysA	Escherichia coli	Silencing
Escherichia coli TH05	metA	Escherichia coli	Silencing
Escherichia coli TH06	ilvA	Escherichia coli	Attenuation
Escherichia coli TH07	tdh	Escherichia coli	Silencing
Escherichia coli TH09C	iclR	Escherichia coli	Silencing
Escherichia coli TH20C	ppc	Escherichia coli	Overexpression
Escherichia coli ILE01	ilvA	Escherichia coli	Feedback insensitive
Escherichia coli ILE02	ilvA	Escherichia coli	Overexpression
Escherichia coli ILE02	ilvE	Escherichia coli	Overexpression
Escherichia coli ILE02	ilvD	Escherichia coli	Overexpression
Escherichia coli ILE02	ilvC	Escherichia coli	Overexpression
Escherichia coli ILE03	lrp	Escherichia coli	Overexpression
pTacilvAIH	ilvA	Escherichia coli	Feedback insensitive
pTacilvAIH	ilvI	Escherichia coli	Feedback insensitive
pTacilvAIH	ilvH	Escherichia coli	Feedback insensitive
pBRthrABCygaZH	thrA	Escherichia coli	Feedback insensitive
pBRthrABCygaZH	thrB	Escherichia coli	Overexpression
pBRthrABCygaZH	thrC	Escherichia coli	Overexpression
pBRthrABCygaZH	ygaZ	Escherichia coli	Overexpression
pBRthrABCygaZH	ygaH	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Carbon source	Time	Scale	Ref.
L-isoleucine	Titer	2.83 g/L	Glucose		Flask	[228]
L-isoleucine	Titer	8.72 g/L	Glucose		Fed-Batch	[228]
acetate	Titer	12.61 g/L	Glucose		Fed-Batch	[228]
L-isoleucine	Volumetric productivity	0.121 g/L/h	Glucose		Fed-Batch	[228]
L-isoleucine	Titer	9.46 g/L	Glucose	60 h	Fed-Batch	[228]
L-isoleucine	Volumetric productivity	0.158 g/L/h	Glucose	60 h	Fed-Batch	[228]
L-isoleucine	Substrate yield	0.14 g/g of sustrate	Glucose	60 h	Fed-Batch	[228]
acetate	Titer	11.34 g/L	Glucose	60 h	Fed-Batch	[228]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Jin Hwan Park, Jae Eun Oh, Kwang Ho Lee, Ji Young Kim & Sang Yup Lee (2012). Rational Design of Escherichia coli for l‑Isoleucine Production. ACS Synthetic Biology.