Escherichia coli W-H19/pM2/pR1
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlysA ΔthrB ΔmetA ΔlacI | ΔldhA ΔadhE ΔpflB | ΔptsG | ΔPgalP::Plac | ΔPppc::Ptrc | ΔiclR ΔarcA | ΔPaspA::Ptrc | ΔPglk::Plac | ΔPasd::Ptrc | ΔatpFH | Plasmid pM2 added | Plasmid pR1 addedBars (|) indicate differences between strains.
Plasmids
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-homoserine | Titer | 12.4 g/L | Flask | [238] | |||
| L-homoserine | Substrate yield | 0.69 mol/mol of substrate | Flask | [238] | |||
| L-homoserine | Volumetric productivity | 0.59 g/L/h | Flask | [238] | |||
| L-homoserine | Titer | 10.5 g/L | Flask | [238] | |||
| L-homoserine | Substrate yield | 0.57 mol/mol of substrate | Flask | [238] | |||
| L-homoserine | Volumetric productivity | 0.55 g/L/h | Flask | [238] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.