Xu, Jianzhong; Yu, Haibo; Chen, Xiulai; Liu, Liming & Zhang, Weiguo (2020). Accelerated Green Process of 2,5-Dimethylpyrazine Production from Glucose by Genetically Modified Escherichia coli. ACS Synthetic Biology, 9(9), 2576-2587.

Strains

• Escherichia coli Thr-6
• Escherichia coli T6-47
• Escherichia coli T6-47-2
• Escherichia coli T6-47-3
• Escherichia coli T6-47-4
• Escherichia coli T6-47-5
• Escherichia coli T6-47-6
• Escherichia coli T6-47-7
• Escherichia coli MG1655
• Escherichia coli T6-47-1

Plasmids

• pEC-XK99E
• pEC-XK99E-tdh_Ec
• pEC-XK99E-tdh_Ec-noxE_Lc
• pEC-XK99E-tdh_Ec-PnoxE_Lc
• pEC-XK99E-tdh_Ec-aao_So- PnoxE_Lc
• pEC-XK99E-tdh_Ec-Paao_So- PnoxE_Lc
• pCDFDuet-P_sod-sstT_Ec
• pCDFDuet-P_vgb-sstT_Ec
• pCDFDuet-1
• pEC-XK99E-thrA^C1034TBC

Metabolite production

• 2,5-Dimethylpyrazine
  Titer
  • 1.43 g/L (Escherichia coli T6-47-7 in Flask)
  • 0.0 mg/L (Escherichia coli T6-47 in Flask)
  • 0.0 mg/L (Escherichia coli T6-47 in Flask)
  • 0.04 g/L (Escherichia coli Thr-6 in Flask)
  • 0.07 g/L (Escherichia coli Thr-6 in Flask)
  • 0.03 g/L (Escherichia coli MG1655 in Flask)
  • 0.0 g/L (Escherichia coli MG1655 in Flask)
  • 0.44 g/L (Escherichia coli T6-47-1 in Flask)
  • 0.67 g/L (Escherichia coli T6-47-3 in Flask)
  • 0.54 g/L (Escherichia coli T6-47-2 in Flask)
  • 0.89 g/L (Escherichia coli T6-47-4 in Flask)
  • 0.477 g/L (Escherichia coli T6-47-5 in Flask)
  • 0.5 g/L (Escherichia coli T6-47-6 in Flask)
  Volumetric productivity
  • 0.715 g/(L*d) (Escherichia coli T6-47-7 in Flask)
• L-threonine
  Titer
  • 11.2 g/L (Escherichia coli T6-47 in Flask)
  • 3.7 g/L (Escherichia coli Thr-6 in Flask)
  • 0.0 g/L (Escherichia coli MG1655 in Flask)
• aminoacetone
  Titer
  • 0.0 g/L (Escherichia coli T6-47 in Flask)
  • 0.0 g/L (Escherichia coli T6-47 in Flask)